Role of Porphyromonas gingivalis gingipains in multi-species biofilm formation

BackgroundPeriodontal diseases are polymicrobial diseases that cause the inflammatory destruction of the tooth-supporting (periodontal) tissues. Their initiation is attributed to the formation of subgingival biofilms that stimulate a cascade of chronic inflammatory reactions by the affected tissue. The Gram-negative anaerobes Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola are commonly found as part of the microbiota of subgingival biofilms, and they are associated with the occurrence and severity of the disease. P. gingivalis expresses several virulence factors that may support its survival, regulate its communication with other species in the biofilm, or modulate the inflammatory response of the colonized host tissue. The most prominent of these virulence factors are the gingipains, which are a set of cysteine proteinases (either Arg-specific or Lys-specific). The role of gingipains in the biofilm-forming capacity of P. gingivalis is barely investigated. Hence, this in vitro study employed a biofilm model consisting of 10 ¿subgingival¿ bacterial species, incorporating either a wild-type P. gingivalis strain or its derivative Lys-gingipain and Arg-gingipan isogenic mutants, in order to evaluate quantitative and qualitative changes in biofilm composition.ResultsFollowing 64 h of biofilm growth, the levels of all 10 species were quantified by fluorescence in situ hybridization or immunofluorescence. The wild-type and the two gingipain-deficient P. gingivalis strains exhibited similar growth in their corresponding biofilms. Among the remaining nine species, only the numbers of T. forsythia were significantly reduced, and only when the Lys-gingipain mutant was present in the biofilm. When evaluating the structure of the biofilm by confocal laser scanning microscopy, the most prominent observation was a shift in the spatial arrangement of T. denticola, in the presence of P. gingivalis Arg-gingipain mutant.ConclusionsThe gingipains of P. gingivalis may qualitatively and quantitatively affect composition of polymicrobial biofilms. The present experimental model reveals interdependency between the gingipains of P. gingivalis and T. forsythia or T. denticola

Effective Immunological Guidance of Genetic Analyses Including Exome Sequencing in Patients Evaluated for Hemophagocytic Lymphohistiocytosis

We report our experience in using flow cytometry-based immunological screening prospectively as a decision tool for the use of genetic studies in the diagnostic approach to patients with hemophagocytic lymphohistiocytosis (HLH). We restricted genetic analysis largely to patients with abnormal immunological screening, but included whole exome sequencing (WES) for those with normal findings upon Sanger sequencing. Among 290 children with suspected HLH analyzed between 2010 and 2014 (including 17 affected, but asymptomatic siblings), 87/162 patients with "full" HLH and 79/111 patients with "incomplete/atypical" HLH had normal immunological screening results. In 10 patients, degranulation could not be tested. Among the 166 patients with normal screening, genetic analysis was not performed in 107 (all with uneventful follow-up), while 154 single gene tests by Sanger sequencing in the remaining 59 patients only identified a single atypical CHS patient. Flow cytometry correctly predicted all 29 patients with FHL-2, XLP1 or 2. Among 85 patients with defective NK degranulation (including 13 asymptomatic siblings), 70 were Sanger sequenced resulting in a genetic diagnosis in 55 (79%). Eight patients underwent WES, revealing mutations in two known and one unknown cytotoxicity genes and one metabolic disease. FHL3 was the most frequent genetic diagnosis. Immunological screening provided an excellent decision tool for the need and depth of genetic analysis of HLH patients and provided functionally relevant information for rapid patient classification, contributing to a significant reduction in the time from diagnosis to transplantation in recent years

Risk governance in organizations

Dieses Buch dokumentiert 10 Jahre Risk-Governance-Forschung an der Universität Siegen. In 50 Beiträgen reflektieren Forscher und Praktiker Risk Governance vor dem Hintergrund ihrer eigenen Forschungen und/oder Erfahrungen und geben jeweils einen Entwicklungsimpuls für die Zukunft der Risk Governance. Das Buch zeigt die große Bandbreite und Tiefe des Forschungsgebietes auf und diskutiert Grundannahmen, Implementierungsfragen, die Rolle der Risk Governance als Transformationsmotor, ihre Wirkung in den verschiedenen betrieblichen Funktionen, Entwicklungsperspektiven und den Beitrag der Risk Governance zu einer nachhaltigen Ausrichtung von Unternehmen.This book documents 10 years of risk governance research at the University of Siegen. In 50 contributions, researchers and practitioners reflect on risk governance against the background of their own research and/or experience and provide a development impetus for the future of risk governance. The book shows the wide range and depth of the research field and discusses basic assumptions, implementation issues, the role of risk governance as transformation engine, its impact in the various operational functions, development perspectives, and the contribution of risk governance to a sustainable orientation of companies

Spectral data fusion for robust ECG-derived respiration with experiments in different physical activity levels

In this paper, we study instant respiratory frequency extraction using single-channel electrocardiography (ECG) during mobile conditions such as high intensity exercise or household activities. Although there are a variety of ECG-derived respiration (EDR) methods available in the literature, their performance during such activities is not very well-studied. We propose a technique to boost the robustness and reliability of widely used and computationally efficient EDR methods, aiming to qualify them for ambulatory and daily monitoring. We fuse two independent sources of respiratory information available in ECG signal, including respiratory sinus arrhythmia (RSA) and morphological change of ECG time series, to enhance the accuracy and reliability of instant breathing rate estimation during ambulatory measurements. Our experimental results show that the fusion method outperforms individual methods in four different protocols, including household and sport activities

Fast and Simple High Resolution Optical Spectrum Analyser

A simple method for fast optical spectroscopy with high resolution is shown. The method is based on the narrowband Brillouin gain process in optical fibers

Slow and Fast-light in Optical Fibers: an Overview

Slow- and fast-light is the control of the velocity of light in a medium by light. As a fascinating new field in physics there is a fundamental interest on this effect on the one side, but on the other side there exist a lot of practical applications for telecommunication and information systems. Among these are optical signal processing, the radio frequency-photonics, nonlinear optics and spectroscopy in time domain. Furthermore, the slow- and fast-light effect can be seen as a key technology for optical delay lines, buffers, equalizers and synchronizers in packed switched networks. To realize the effect there are different methods and material systems possible. Beside these especially the nonlinear effect of stimulated Brillouin scattering (SBS) is of special interest because it has several advantages. This article gives an overview about the fundamentals and limits of the slow-and fast-light effect in general and based on the SBS in optical fibers. Some experimental results which were achieved so far are shown

1 Gbit/s Radio Over Fiber Downlink at a 32 GHz Carrier

The carrier frequency of the presented radio over fiber downlink at 32 GHz is generated by stimulated Brillouin scattering and an error free 1 Gbit/s data transmission is realized

A Comparative Test of Brillouin Amplification and Erbium-doped Fiber Amplification for the Generation of Millimeter Waves with Low Phase Noise Properties

Measurements of phase noise of a 40-GHz carrier signal are presented. The carrier is generated by the amplification of harmonics due to stimulated Brillouin scattering. An analogy to a generation of millimeter waves by an erbium-doped fiber instead of the Brillouin amplifier is investigated and discussed. In our setup, both show a comparable behavior in respect to their noise characteristics

Investigation of Fast Light in Long Optical Fibers Based on Stimulated Brillouin Scattering

A simple method to generate a negative time delay in SBS-based fast-light systems using Brillouin gain and loss is shown. We achieved a maximum negative time delay of 32.4 ns in one long fiber segment

Optimization of the Brillouin Spectrum for Fiber Based Slow Light Systems

This article shows simulation results and first practical investigations of the optimization of a Brillouin spectrum with the natural bandwidth superimposed with two losses for fiber based slow light systems